VitaFast Cystin

VitaFast^® L-Cystin

Product Code P1013

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of the
added L-cystine or L-cysteine and the total content (added L-cystine or
L-cysteine and natural L-cysteine) in food animal feed.

 General information:

L-cystine and L-cysteine is extracted from the sample and the extract is
diluted. The L-cystine assay - medium and the diluted extract are pipetted into
the wells of a microtiter plate which is coated with Pediococuss acidilactici.
The growth of Pediococcus acidilactici is dependent on the supply of
L-cystine or L-cysteine. Following the addition of L-cystine as a standard or
as a compound of the sample, the bacteria grow until the L-cystine and L-cysteine is consumed. The incubation is done in the dark at 37 °C (98.6 °F)
for 44 - 48 h.
The intensity of metabolism or growth of Pediococcus acidilactici in relation to
the extracted L-cystine or L-cysteine is measured as turbidity and compared
to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: