VitaFast Lysin

VitaFast^® L-Lysin

Product Code P1012

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of free
L-lysine (free and bound L-lysine) in animal feed.

 General information:

L-lysine is extracted from the sample and the extract is diluted. The diluted
extract and the L-lysine assay - medium are pipetted into the wells of a
microtiter plate which is coated with Pediococuss acidilactici. The growth of Pediococcus acidilactici is dependent on the supply of L-lysine. Following the addition of L-lysine as a standard or as a compound of the sample, the bacteria grow until the L-lysine is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 48 h.
The intensity of metabolism or growth of Pediococcus acidilactici in relation to
the extracted L-lysine is measured as turbidity and compared to a standard
curve. The measurement is done using a microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: