VitaFast Methionin

VitaFast^® D-/L-Methionin

Product Code P1011

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of the
added, free DL-methionine and the protein bound, natural L-methionine in
animal feed.

 General information:

Methionine is extracted from the sample and the extract is diluted. The diluted
extract and the methionine assay - medium are pipetted into the wells of a
microtiter plate which is coated with Pediococuss acidilactici. The growth of Pediococcus acidilactici is dependent on the supply of L-methionine. Following the addition of L-methionine as a standard or as a
compound of the sample, the bacteria grow until the DL-methionine is
consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 48 h.
The intensity of metabolism or growth of Pediococcus acidilactici in relation to
the extracted L-methionine is measured as turbidity and compared to a
standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: