Ridascreen Verotoxin

RIDASCREEN^® Verotoxin

Product Code R5701

 Bruksområde:

RIDASCREEN^® Verotoxin er et enzyme immunoassay for deteksjon av verotoxin 1 og 2 (SLT-I og SLT-II) fra en oppformeringsbuljong.

 Generell informasjon:

Enterobacteriacea of the species Escherichia coli (E. coli) are part of the intestinal
flora of healthy humans, as well as many farm animals. Therefore, they are
indicators of fecal contamination of water and food.
Due to different pathogenicity factors, often plasmid-coded or transmitted by
phages, E. coli are considered as facultative pathogenic. Since 1977, E. coli are
known which are able to produce two cytotoxins, verotoxin 1 and 2.
Due to the similarities of the verotoxins to the shiga toxin of shigella dysentery,
they have also been labeled as the shiga-like toxin 1 and 2 (SLT-I and SLT-II).
The "prototype" of enterohemorrhagic E. coli (EHEC) was first described in 1982.
The clinical symptoms which are caused by EHEC range from light to severe
gastroenteritis to hemorrhagic colitis which occurs in 10 - 20 % of all infections.
Also life-threatening and post-infectious complications in 5 - 10 % of the infections
can occur. Especially infants and small children, but also old patients or patients
with a weakened immune system are affected. Due to its high environmental resistance and relative acid tolerance, the infection level for EHEC is only around
10²2 microbes. Main sources of infection are products from cattle, sheep and goats,
especially raw or insufficiently cooked meat and poorly stored meat products and
raw milk. Chains of infection from human to human, especially in public institutions
such as child care centers, senior citizen homes or hospitals, as well as direct
contact to animals are of importance.
Today's recommended procedure for food analysis consists of a pre-enrichment
of the pathogen for 6 h, inoculation of an aliquot of the pre-enrichment into a fresh
broth and subsequently incubation overnight (16 - 18 h). After that the bacteria
should be isolated. At the same time, as the safest pathenogenicity factor,
verotoxin detection should be carried out using the enrichment broth. This can be
done by means of PCR or immunologically by ELISA. In comparison to PCR,
ELISA techniques such as the RIDASCREEN^® Verotoxin assay, are simple
methods which are practical for any laboratory. An enrichment of the germs is
required for both methods since the germ excretion as well as the toxin content in
food can be very low.

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