VitaFast Inositol

VitaFast^® Inositol

Product Code P1009

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of total
inositol (added and native inositol) in food, animal feed and pharmaceutical
products.

 General information:

Inositol is extracted from the sample and the extract is diluted. The diluted
extract and the inositol assay - medium are pipetted into the wells of a
microtiter plate which is coated with Saccharomyces cerevisiae. The growth
of Saccharomyces cerevisiae is dependent on the supply of inositol.
Following the addition of inositol as a standard or as a compound of the
sample, the bacteria grow until the inositol is consumed. The incubation is
done in the dark at 30 °C (86 °F) for 44 - 48 h.
The intensity of metabolism or growth of Saccharomyces cerevisiae in
relation to the extracted inositol is measured as turbidity and compared to a
standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: