VitaFast Vitamin B2(Riboflavin)

VitaFast® Vitamin B2 (Riboflavin)

Product Code P1007

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of total vitamin B2 (enriched and natural vitamin B2) in food, feed and pharmaceutical products.

 General information:

Vitamin B2 is extracted from the sample and the extract is diluted. The diluted
extract and the vitamin B2 assay-medium are pipetted into the wells of a
microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of vitamin B2. Following the addition of vitamin B2 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed.
The incubation is done in the dark at 37 °C (98.6 °F) for 44 - 48 h.
The intensity of metabolism or growth in relation to the extracted vitamin B2 is measured as turbidity and compared to a standard curve. The measurement
is done using a microtiter plate spectrophotometer at 610 - 630 nm (alternatively at
540 - 550 nm).

 Specifications: