VitaFast Vitamin B3 (Niacin)

VitaFast^® Vitamin B3 (Niacin)

Product Code P1004

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of total niacin (enriched and natural niacin) in food, feed and pharmaceutical
products. All required reagents and standard are contained in the test kit.

 General information:

Niacin is extracted from the sample and the extract is diluted. The niacin
assay - medium and the diluted extract are pipetted into the wells of a
microtiter plate which is coated with Lactobacillus plantarum. The growth of
Lactobacillus plantarum is dependent on the supply of niacin. Following the
addition of niacin as a standard or as a compound of the sample, the bacteria
grow until the vitamin is consumed. The incubation is done in the dark at
37 °C (98.6 °F) for 44 - 48 h.
The intensity of metabolism or growth of Lactobacillus plantarum in relation to
the extracted niacin is measured as turbidity and compared to a standard
curve. The measurement is done using an microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: