VitaFast Vitamin B5 (Pantotensyre)

VitaFast^® Pantothensäure / Pantothenic Acid

Product Code P1005

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of total pantothenic acid (enriched and natural pantothenic acid) in food, feed and pharmaceutical products.

 General information:

Pantothenic acid is extracted from the sample and the extract is diluted. The
diluted extract and the pantothenic acid assay-medium are pipetted into the
wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply
of pantothenic acid. Following the addition of pantothenic acid as a standard
or as a compound of the sample, the bacteria grow until the vitamin is
consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 20 - 24 h.
The intensity of metabolism or growth in relation to the extracted pantothenic
acid is measured as turbidity and compared to a standard curve. The
measurement is done using an microtiter plate spectrophotometer at 610 - 630 nm (alternatively at 540 - 550 nm).

 Specifications: