VitaFast Vitamin B7 (Biotin)

VitaFast^® Vitamin B7 (Biotin)

Product Code P1003

 Intended use:

Easy to use microbiological microtiter plate test for the quantification of total biotin (enriched and natural biotin) in food, feed and pharmaceutical products.

 General information:

Biotin is extracted from the sample and the extract is diluted. The diluted
extract and the biotin assay-medium are pipetted into the wells of a microtiter
plate which is coated with Lactobacillus plantarum . The growth of Lactobacillus plantarum is dependent on the supply of biotin.
Following the addition of biotin as a standard or as a compound of the
sample, the bacteria grow until the vitamin is consumed. The incubation is
done in the dark at 37 °C (98.6 °F) for 44 - 48 h.
The intensity of metabolism or growth in relation to the extracted biotin is
measured as turbidity and compared to a standard curve. The measurement
is done using an microtiter plate spectrophotometer at 610 - 630 nm (alternatively at
540 - 550 nm).

 Specifications: